ผู้ช่วยศาสตราจารย์ ดร.ทนพญ.ภัทริน ตั้งธนตระกูล

RS_pattarin-tangtanatakul

Education

Autoimmune molecular and Red Blood Cell genotyping

Training

Department of Microbiology, Faculty of Medicine, Chulalongkorn University

• Separated Neutrophils from healthy donors and culture neutrophils with ascorbic acid (Vitamin C)
• Examined Neutrophils phagocytosis and foreign antigens killing capacity in two conditions (Vitamin C stimulation and Mock control)
• Studied repetitive sequences function and identified epigenetics regulation of repetitive sequences
• Extracted small non-coding RNA (microRNA) from human skin with psoriasis and healthy individuals
• Detected microRNAs expression in the psoriasis patients compared with controls using stem-loop RT-PCR and qPCR
• Determined microRNAs expression in serum from sepsis-induced encephalopathy mouse model
• Explore the immunological training and tolerance of professional antigen presenting in Fc-gamma receptor knockout mice
• Verified mRNA expression in the skin from Discoid Lupus Erythematosus (DLE) patients before and after UVB treatment Bioinformatics analysis (1 year) Aug 2016 – Sep 2017

Department of Pediatrics and Adolescent, Hong Kong University

• Unix Operating systems coding
• Basic Perl and Python programming
• R-bioconductor : lumi package, egdeR packages, biomaRt packages and lima packages, qqman package, suerat package
• RNA-Seq analysis pipelines including FastQC, picard, STAR aligner, HT￾Seq, RNA-SeqQC, bedtools and samtools
• Repetitive sequences analysis in PBMC from systemic lupus erythematosus patients RNA-Seq data
• RNA-Seq analysis in Notch1 signaling knockout mice (Notch are crucial for cell development and inflammatory response)
• RNA-Seq analysis in human THLE-2 transfected cells compared to scramble controls cells (hepatocellular carcinoma)
• Small RNA-Seq analysis using miRDeep2 and sRNAbench program

Professional Experience

Chulalongkorn University Instructor Jan 2019 – Present

Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University

• The functional and identification of STAT4 in purified human Dendritic cells (DC) in Systemic Lupus Erythematosus patients
• X chromosome-wide association study in Systemic Lupus Erythematosus patients
• Red blood cell antigens genotyping
• Proteomic study in Hair loss

Bioinformatic and Researcher assistant (4 years) Jan 2014 –Dec 2018

Publications

1. Boonpethkaew S, Meephansan J, Jumlongpim O, Charoensuksira S, Tangtanatakul P, Wongpiyabovorn J, Komine M. Comparison of transcriptomic profiles in edge to center of plaque reveals chronological molecular events in psoriatic plaque formation. J Dermatol Sci. 2022 Oct 13:S0923-1811(22)00243-2. doi:10.1016/j.jdermsci.2022.10.003. Epub ahead of print. PMID: 36307341. Transcriptomic study
2. Vacharanukrauh P, Meephansan J, Ponnikorn S, Tangtanatakul P, Soonthornchai W, Wongpiyabovorn J, Ingkaninanda P, Morita A. Transcriptome profiling in psoriasis: NB-UVB treatment-associated transcriptional changes and modulation of autoinflammation in perilesional skin in early-phase disease. J Dermatol Sci. 2022 Sep;107(3):123-132. doi: 10.1016/j.jdermsci.2022.08.004. Epub 2022 Aug 12. PMID: 35995712. Transcriptomic study
3. Hiengrach P, Visitchanakun P, Tongchairawewat P, Tangsirisatian P, Jungteerapanich T, Ritprajak P, Wannigama DL, Tangtanatakul P, Leelahavanichkul A. Sepsis Encephalopathy Is Partly Mediated by miR370-3p￾Induced Mitochondrial Injury but Attenuated by BAM15 in Cecal Ligation and Puncture Sepsis Male Mice. Int J Mol Sci. 2022 May 13;23(10):5445. doi: 10.3390/ijms23105445. PMID: 35628259; PMCID: PMC9141734. Transcriptomic study
4. Boonpethkaew S, Meephansan J, Jumlongpim O, Tangtanatakul P, Soonthornchai W, Wongpiyabovorn J, Vipanurat R, Komine M. Transcriptomic Profiling of Peripheral Edge of Lesions to Elucidate the Pathogenesis of Psoriasis Vulgaris. Int J Mol Sci. 2022 Apr 30;23(9):4983. doi: 10.3390/ijms23094983.
5. Vacharanukrauh P, Meephansan J, Tangtanatakul P, Soonthornchai W, Wongpiyabovorn J, Serirat O, Komine M. High-Throughput RNA Sequencing Reveals the Effect of NB-UVB Phototherapy on Major Inflammatory Molecules of Lesional Psoriasis. Psoriasis (Auckl). 2021 Nov 26;11:133-149. doi: 10.2147/PTT.S335913. PMID: 34858799; PMCID: PMC8631988. Transcriptomic study
6. Wang YF, Wei W, Tangtanatakul P, Zheng L, Lei Y, Lin Z, Qian C, Qin X, Hou F, Zhang X, Shao L, Satproedprai N, Mahasirimongkol S, Pisitkun P, Song Q, Lau YL, Zhang Y, Hirankarn N, Yang W. Identification of Shared and Asian-Specific Loci for Systemic Lupus Erythematosus and Evidence for Roles of Type III Interferon Signaling and Lysosomal Function in the Disease: A Multi-Ancestral Genome￾Wide Association Study. Arthritis Rheumatol. 2022 May;74(5):840-848. doi: 10.1002/art.42021. Epub 2022 Mar 29. PMID: 34783190. GWAS analysis and meta-analysis
7. Soonthornchai W, Tangtanatakul P, Meesilpavikkai K, Dalm V, Kueanjinda P, Wongpiyabovorn J. MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene. Sci Rep. 2021 Jul 9;11(1):14186. doi: 10.1038/s41598-021-93616-8. PMID: 34244572; PMCID: PMC8270917. Transcriptomic study
8. Tangtanatakul P, Thumarat C, Satproedprai N, Kunhapan P, Chaiyasung T, Klinchanhom S, Wang YF, Wei W, Wongshinsri J, Chiewchengchol D, Rodsaward P, Ngamjanyaporn P, Suangtamai T, Mahasirimongkol S, Pisitkun P, Hirankarn N. Meta-analysis of genome-wide association study identifies FBN2 as a novel locus associated with systemic lupus erythematosus in Thai population. Arthritis Res Ther. 2020 Aug 8;22(1):185. doi: 10.1186/s13075-020-02276-y. PMID: 32771030; PMCID: PMC7414652. GWAS analysis and meta-analysis
9. Saejong S, Townamchai N, Somparn P, Tangtanatakul P, Ondee T, Hirankarn N, Leelahavanichkul A. MicroRNA-21 in plasma exosome, but not from whole plasma, as a biomarker for the severe interstitial fibrosis and tubular atrophy (IF/TA) in post-renal transplantation. Asian Pac J Allergy Immunol. 2022 Mar;40(1):94-102. doi: 10.12932/AP-101019-0656. PMID: 32563236. Epigenetics
10. Visitchanakun P, Tangtanatakul P, Trithiphen O, Soonthornchai W, Wongphoom J, Tachaboon S, Srisawat N, Leelahavanichkul A. Plasma miR-370- 3P as a Biomarker of Sepsis-Associated Encephalopathy, the Transcriptomic Profiling Analysis of Microrna-Arrays From Mouse Brains. Shock. 2020 Sep;54(3):347-357. doi: 10.1097/SHK.0000000000001473. PMID: 31743302. Transcriptomic study and Epigenetics
11. Soonthornchai W, Tangtanatakul P, Meephansan J, Ruchusatsawat K, Reantragoon R, Hirankarn N, Wongpiyabovorn J. Down-regulation of miR-155 after treatment with narrow-band UVB and methotrexate associates with apoptosis of keratinocytes in psoriasis. Asian Pac J Allergy Immunol. 2021 Sep;39(3):206-213. doi:10.12932/AP-031218-0451. PMID: 30904000. Transcriptomic study
12. Kongkavitoon P, Butta P, Sanpavat A, Bhattarakosol P, Tangtanatakul P, Wongprom B, Tangkijvanich P, Hirankarn N, Palaga T. Regulation of periostin expression by Notch signaling in hepatocytes and liver cancer cell lines. Biochem Biophys Res Commun. 2018 Nov 30;506(3):739-745. doi: 10.1016/j.bbrc.2018.10.144. Epub 2018 Oct 30. PMID: 30384995. Transcriptomic study
13. Tangtanatakul P, Klinchanhom S, Sodsai P, Sutichet T, Promjeen C, Avihingsanon Y, Hirankarn N. Down-regulation of let-7a and miR-21 in urine exosomes from lupus nephritis patients during disease flare. Asian Pac J Allergy Immunol. 2019 Dec;37(4):189-197. doi: 10.12932/AP-130318-0280. PMID: 30118248. Epigenetics study
14. Surawut S, Panpetch W, Makjaroen J, Tangtanatakul P, Thim-Uam A, Wongphoom J, Tumwasorn S, Leelahavanichkul A. <i>Helicobacter pylori</i> Infection Increased Anti-dsDNA and Enhanced Lupus Severity in Symptomatic FcγRIIb-Deficient Lupus Mice. Front Microbiol. 2018 Jul 6;9:1488. doi: 10.3389/fmicb.2018.01488. PMID: 30034379; PMCID: PMC6043646. Animal model
15. Wongchana W, Kongkavitoon P, Tangtanatakul P, Sittplangkoon C, Butta P, Chawalitpong S, Pattarakankul T, Osborne BA, Palaga T. Notch signaling regulates the responses of lipopolysaccharide-stimulated macrophages in the presence of immune complexes. PLoS One. 2018 Jun 11;13(6):e0198609. doi: 10.1371/journal.pone.0198609. PMID: 29889863; PMCID: PMC5995379. Transcriptomic study
16. Rerknimitr P, Tekacharin N, Panchaprateep R, Wititsuwannakul J, Tangtanatakul P, Hirankarn N, Asawanonda P. Pulsed-dye laser as an adjuvant treatment for discoid lupus erythematosus: a randomized, controlled trial. J Dermatolog Treat. 2019 Feb;30(1):81-86. doi: 10.1080/09546634.2018.1468063. Epub 2018 May 10. PMID: 29676592. Clinical trial
17. Tangtanatakul P, Thammasate B, Jacquet A, Reantragoon R, Pisitkun T, Avihingsanon Y, Leelahavanichkul A, Hirankarn N. Transcriptomic profiling in human mesangial cells using patient-derived lupus autoantibodies identified miR-10a as a potential regulator of IL8. Sci Rep. 2017 Nov 6;7(1):14517. doi: 10.1038/s41598-017-15160-8. PMID: 29109423; PMCID: PMC5673966. Transcriptomic study